The Advanced Quality One Step Malaria (P.F) Test is a colloidal gold enhanced immunoassay for the determination of plasmodium falciparum malaria in human whole blood2. A monoclonal antibody (anti-HRP II) is immobilized in the test region on nitrocellulose membrane. During the assay the HRP-11 which is released by lysed red blood cells are selectively to react with the colored conjugate (antibody-colloidal gold conjugate); the mixture then migrates chromatographically on the membrane by the capillary action. A plasmodium falciparum malaria positive sample produces a distinct color band in the test region, formed by the specific antibody-plasmodium falciparum malaria-colored conjugate complex. Absence of this colored band in the test region suggests a negative result. A colored band always appears in the control region serving as procedural control regardless of the test result.