Dengue virus belonging to the Flavavirus group of viruses, is one of the most significant mosquito-born diseases in the world in terms of morbidity and mortality. Transmitted principally by the mosquito types Aedes aegypti and Aedes albopictus, the virus is found commonly throughout the tropic and subtropic regions of the world.

There are four known serotypes of dengue. Symptoms of dengue fever include high fever, headache, muscle pain and skin rash. The complications often associated with this infection are dengue hemorrhagic fever or dengue shock syndrome.There are four known distinct serotypes(dengue virus 1,2,3 and 4).NS1 is a highly conserved glycoprotein that is present at high concentrations in the sera of dengue. Infected patients during the early clinical phase of the disease.NS1 antigen is found from the first day up to 9 days after onset of fever in sample of primary or secondary dengue infected patients.